Japan's Security Policy

Abstract
We are developing methods to image her-2-neu oncogene over-expression in breast cancer using positron emission tomography (PET) . Small oligodeoxynucleotides (ODNs) that are complementary to the Her-2-neu messenger RNA (mRNA) are being investigated as potential imaging probes. Fluorine-l8 (2 hour half-life positron emitter) has been used to label 15-18 mer ODN probes. Furthermore, several breast and ovarian cancer cell lines which over-express ODNs have been used to study trapping of 1SF- ODNs in cell culture. We have also preliminary studied the biodistribution of ODNs in 2 living nude mice using micropET in order to understand the limitations of imaging in vivo with the antisense approach. Currently the yield of radiolabeled ODNs is very low (20 uci), and therefore more work will have to be performed to optimize yields of the ODNs prior to proceeding further. Once ODN yields can be brought into the 150-300 uCi range, then more cell culture and in vivo experiments will be able to be performed to optimize an anti sense based approach for imaging breast cancer.

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