Ex vivo Vascular Perfusion of the Isolated Canine Pancreas

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Abstract
Based on a previous experience of 130 ex vivo canine pancreatic perfusions, a new experimental procedure, dealing with 15 experiments, is reported. Viability and functional responses of the preparation have been improved by the prevention of ischaemia and the use of a pulsatile flow without recirculation of the perfusate. The perfusate was a suspension of homologous erythrocytes in a culture medium. Haemodynamic, biochemical and biological parameters were analysed and compared to those obtained in intact animals. Endocrine behaviour of the perfused pancreas was studied in relation with arterial glucose concentration: less than 1.40 g/l of glycaemia did not induce an insulin secretion, but this was induced and increased with increasing glucose concentration. On the other hand, the exocrine response to prolonged secretin and secretin + CCK infusion was investigated. Both endocrine and exocrine functional responses of the perfused pancreas attested the good survival for a definite time of such an ex vivo preparation.

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