Detection of Mycoplasma pneumoniae in simulated clinical samples by Polymerase Chain Reaction
- 1 July 1989
- Vol. 97 (7-12) , 1046-1048
- https://doi.org/10.1111/j.1699-0463.1989.tb00516.x
Abstract
Polymerase chain reaction (PCR) was used to detect Mycoplasma (M) pneumoniae DNA in simulated clinical samples. Throat swabs were mixed with known amounts of broth-grown M. pneumoniae cells. An estimated detection limit of less than 40 colony forming units (cfu) was obtained without the need for time-consuming hybridization. The PCR is completed in one day and may be useful for the early detection of M. pnuemoniae in clinical samples.Keywords
This publication has 9 references indexed in Scilit:
- Polymerase Chain ReactionThe Journal of Infectious Diseases, 1988
- Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tractJournal of Clinical Microbiology, 1988
- Laboratory diagnosis ofMycoplasma pneumoniaeinfection: 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay.Epidemiology and Infection, 1988
- Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniaeGene, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniaeJournal of Clinical Microbiology, 1984
- UREA HYDROLYSISPublished by Elsevier ,1983