Mapping molecular landscapes inside cells
- 1 October 2004
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 385 (10) , 865-872
- https://doi.org/10.1515/bc.2004.113
Abstract
Cryoelectron tomography opens a window into the inner space of cells. It combines the potential of three-dimensional imaging with a close-to-life preservation of biological samples. Tomograms with molecular resolution are essentially images of the cellular proteome and, in conjunction with advanced pattern recognition techniques, they can be used to map the molecular landscape inside organelles and cells.Keywords
This publication has 31 references indexed in Scilit:
- Identification of macromolecular complexes in cryoelectron tomograms of phantom cellsProceedings of the National Academy of Sciences, 2002
- Towards automatic electron tomographyPublished by Elsevier ,2002
- Noise Reduction in Electron Tomographic Reconstructions Using Nonlinear Anisotropic DiffusionJournal of Structural Biology, 2001
- FhuA-mediated phage genome transfer into liposomesCurrent Biology, 2001
- Toward detecting and identifying macromolecules in a cellular context: Template matching applied to electron tomogramsProceedings of the National Academy of Sciences, 2000
- Electron tomography of molecules and cellsTrends in Cell Biology, 1999
- Zero‐loss energy filtering under low‐dose conditions using a post‐column energy filterJournal of Microscopy, 1996
- Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomographyBiophysical Journal, 1995
- Cryo-electron microscopy of vitrified specimensQuarterly Reviews of Biophysics, 1988
- Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron MicrographsNature, 1970