QUANTITATIVE AUTORADIOGRAPHY WITH RADIOPHARMACEUTICALS .1. DIGITAL FILM-ANALYSIS SYSTEM BY VIDEODENSITOMETRY - CONCISE COMMUNICATION
- 1 January 1983
- journal article
- research article
- Vol. 24 (3) , 231-237
Abstract
A simple low-cost digital film-analysis system using videodensitometry was developed to quantitate autoradiograms of mice. It is based on a TV-film analysis system coupled to a minicomputer. Digital sampling of transmitted light intensities through the autoradiogram is performed with 8-bit gray levels according to the selected array size (128 .times. 128 to 1024 .times. 1024). The performance characteristics of the system provide sufficient stability, uniformity, linearity and intensity response for use in quantitative analysis. Digital images of the autoradiograms are converted to radioactivity content, pixel by pixel, using step-wedge standards. This type of low-cost system can be installed on conventional minicomputers commonly used in modern nuclear medical facilities. Quantitative digital autoradiography can play an important role, with applications stretching from dosimetry calculations of radiopharmaceuticals [14C-methylmethacrylate and 14C-2-deoxy-D-glucose] to metabolic studies in conjunction with positron-emission tomography. [Activity was measured in liver, heart, blood pool and muscle.].This publication has 3 references indexed in Scilit:
- QUANTITATIVE AUTORADIOGRAPHY WITH RADIOPHARMACEUTICALS .2. APPLICATIONS IN RADIOPHARMACEUTICAL RESEARCH - CONCISE COMMUNICATION1983
- Standardization and calibration of whole-body autoradiography for routine semiquantitative analysis of the distribution of 14C-labeled compounds in animal tissuesToxicology and Applied Pharmacology, 1981
- THE [14C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT1Journal of Neurochemistry, 1977