Container- and nursery-grown black oak seedlings inoculated with Pisolithustinctorius: growth and ectomycorrhizal development during seedling production period

Abstract
Vegetative mycelial inoculum of Pisolithustinctorius was used to inoculate black oak (Quercusvelutina Lam.) seedlings grown in containers in a greenhouse and in a fumigated soil in a southern Missouri nursery. Evaluations of seedlings in the greenhouse and the nursery at the end of the first growing season revealed that Pisolithus formed ectomycorrhizae on approximately 40% of the lateral roots. In the nursery, seedling dry weight, shoot length, root collar diameter, and leaf area were significantly greater for inoculated seedlings than for noninoculated control seedlings. For container-grown seedlings, total dry weight, root dry weight, and leaf surface area were significantly increased by inoculation with Pisolithus. These results demonstrate that Pisolithus can be artifically introduced into fumigated nursery soil and sterile growth medium of containers and form ectomycorrhizae on black oak seedlings under greenhouse and nursery conditions.
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