Vascular anticoagulant β: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity
- 1 October 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 185 (1) , 63-71
- https://doi.org/10.1111/j.1432-1033.1989.tb15082.x
Abstract
A cDNA was cloned coding for a new member of the human Ca2+-modulated phospholipid-binding protein family termed annexins. Due to its 56% identity to the human vascular anticoagulant (VAC) the new protein is named VAC-.beta., renaming the previous VAC as VAC-.alpha.. Northern analysis detects one hybridizing mRNA species of 2.2 kb in human placenta. Genomic Southern blot analysis shows a VAC-.beta. gene of comparable complexity to the VAC-.alpha. gene. The cDNA was expressed in Escherichia coli and the recombinant protein purified to homogeneity. Antiserum raised against VAC-.beta. weakly cross-reacts with VAC-.alpha.. The properties of VAC-.beta. as an anticoagulant and as an inhibitor of phospholipase A2 activity were analyzed and compared to those of VAC-.alpha.This publication has 39 references indexed in Scilit:
- Purification and characterization of a novel protein from bovine aorta that inhibits coagulationEuropean Journal of Biochemistry, 1988
- Antibodies to the N-terminus of calpactin II (p35) affect calcium binding and phosphorylation by the epidermal growth factor receptor in vitroBiochemistry, 1988
- Common domain structure of Ca2+ and lipid‐binding proteinsFEBS Letters, 1986
- Rapid and Sensitive Protein Similarity SearchesScience, 1985
- Construction of expression plasmids producing high levels of human leukocyte-type interferon in Escherichia coliGene, 1983
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Blood CoagulationAnnual Review of Biochemistry, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Bovine factor X1 (Stuart factor). Mechanism of activation by a protein from Russell's viper venomBiochemistry, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970