Immunogenicity of rice dwarf virus-ribonucleic acid.

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Abstract
Using rice dwarf virus(RDV)-RNA extracted from RDV and further purified by MAK[methylated albumin Kieselguhr]-column chromatography, anti-RDV-RNA antibodies were produced in rabbits immunized with RDV-RNA-methylated bovine serum albumin complexes. The antisera, as analyzed by complement fixation, cross-reacted with synthetic double stranded RNA [poly (A).cntdot.poly (U), poly (I).cntdot.poly (C)], but not with native or denatured DNA, rRNA, tRNA, 5 S RNA and nucleic acids from rice plants. RDV-RNA treated with heat or dimethylsulfoxide was markedly reduced in reactivity to the antisera. When RDV-RNA was digested with RNase A at low salt concentration, its complement fixation activity was abolished. In double diffusion tests, 2 different precipitation lines were demonstrated between the antiserum and RDV-RNA. One of the precipitation lines connected with these was formed between the antisera, poly (A).cntdot.poly (U) and poly (I).cntdot.poly (C). As regards immunoglobulin [Ig] classes of the antibodies, 1 of the 2 rabbits employed had antibody activity only in IgM and the other, exclusively in IgG.