Structure of the mitochondrial β‐ketoacyl‐[acyl carrier protein] synthase from Arabidopsis and its role in fatty acid synthesis
- 14 October 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 577 (1-2) , 170-174
- https://doi.org/10.1016/j.febslet.2004.10.007
Abstract
Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial β‐ketoacyl‐[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzyme accomplishes the vital condensation steps in constructing fatty acid carbon skeletons. The product profile of mtKAS is unusual in that C8 and C14–16 fatty acyl chains predominate. An enzyme architecture that likely is the basis for the observed bimodal profile of mtKAS products can be derived from the shape of the acyl binding pocket.Keywords
This publication has 30 references indexed in Scilit:
- Cloning, Expression, Characterization, and Interaction of Two Components of a Human Mitochondrial Fatty Acid SynthasePublished by Elsevier ,2003
- Candida tropicalis Expresses Two Mitochondrial 2-Enoyl Thioester Reductases That Are Able to Form Both Homodimers and HeterodimersJournal of Biological Chemistry, 2003
- Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesisPublished by Elsevier ,2003
- ARP⧸wARP and Automatic Interpretation of Protein Electron Density MapsPublished by Elsevier ,2003
- Candida tropicalis Etr1p andSaccharomyces cerevisiae Ybr026p (Mrf1′p), 2-Enoyl Thioester Reductases Essential for Mitochondrial Respiratory CompetenceMolecular and Cellular Biology, 2001
- β-Ketoacyl-[Acyl Carrier Protein] Synthase I of Escherichia coli: Aspects of the Condensation Mechanism Revealed by Analyses of Mutations in the Active Site PocketBiochemistry, 2001
- The crystal structure of β-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54 Å resolution and its relationship to other condensing enzymes11Edited by R. HuberJournal of Molecular Biology, 2001
- The X‐ray crystal structure of β‐ketoacyl [acyl carrier protein] synthase IFEBS Letters, 1999
- The Characterization of a Mitochondrial Acyl Carrier Protein Isoform Isolated from Arabidopsis thalianaPlant Physiology, 1994
- Acyl carrier protein is present in the mitochondria of plants and eucaryotic micro-organismsEuropean Journal of Biochemistry, 1989