MUGOSUBSTANCE HISTOCHEMISTRY OF THE NORMAL MUCOSA and EPITHELIAL NEOPLASMS OF THE LARGE INTESTINE

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Abstract
Histochemical properties of mucosubstances in normal mucosa and neoplasms of 100 operated cases with colonic carcinoma were investigated by using several new methods, such as paradoxical concanavalin A (ConA) staining and modified PAS [periodic acid-Schiff] reactions for sialic acids. Acidity of goblet cell-type, mucin (GCM) of the normal mucosa varied with the depth of the crypt, as well as with different segments of the large intestine, whereas surface coat-type mucin (SCM) mainly consisted of sulfomucin throughout the large intestine. In addition, the PAS reactivity revealing the presence of O-acetylated sialic acid and the labile class III Con A reactivity were demonstrated as hallmarks characterizing the colonic GCM. In carcinoma tissues, sialomucin was more abundant than in the normal mucosa. Goblet-type tumor cells were found in 59 cases. Moreover, O-acetylated sialic acid and the labile class III Con A reactivity persisted in GCM of the goblet-type tumor cells. GCM of the adjacent mucosa of the neoplasms showed a decrease in sulfomucin in 95 cases and a marked increase in the labile class III Con A reactivity in 97 cases, while the histochemical properties of SCM in this region remained unchanged.