Expression of Antisense RNA Targeted against Streptococcus thermophilus Bacteriophages

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Abstract
Antisense RNA complementary to a putative helicase gene ( hel3.1 ) of a cos -type Streptococcus thermophilus bacteriophage was used to impede the proliferation of a number of cos -type S. thermophilus bacteriophages and one pac -type bacteriophage. The putative helicase gene is a component of the Sfi21-type DNA replication module, which is found in a majority of the S. thermophilus bacteriophages of industrial importance. All bacteriophages that strongly hybridized a 689-bp internal hel3.1 probe were sensitive to the expression of antisense hel3.1 RNA. A 40 to 70% reduction in efficiency of plaquing (EOP) was consistently observed, with a concomitant decrease in plaque size relative to that of the S. thermophilus parental strain. When progeny were released, the burst size was reduced. Growth curves of S. thermophilus NCK1125, in the presence of variable levels of bacteriophage κ3, showed that antisense hel3.1 conferred protection, even at a multiplicity of infection of approximately 1.0. When the hel3.1 antisense RNA cassette was expressed in cis from the κ3-derived phage-encoded resistance (PER) plasmid pTRK690:: ori3.1 , the EOP for bacteriophages sensitive to PER and antisense targeting was reduced to between 10 −7 and 10 −8 , beyond the resistance conferred by the PER element alone (less than 10 −6 ). These results illustrate the first successful applications of antisense RNA and explosive delivery of antisense RNA to inhibit the proliferation of S. thermophilus bacteriophages.