Measurement of relative amounts of phospho‐ and dephospho‐B‐50(GAP‐43) peptides by fast atom bombardment‐mass spectrometry
- 20 April 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 301 (2) , 150-154
- https://doi.org/10.1016/0014-5793(92)81236-f
Abstract
The biological role of phosphoproteins depends upon their degree of phosphorylation in vivo. Methods currently available to measure the degree of phosphorylation of a protein involve indirect procedures to detect the 32P-phosphate incorporation. We report here a direct method to measure relative amounts of phospho- and dephospho-forms of peptides based upon a mass spectrometric technique. The intensities of the molecular ions corresponding to the two forms of the peptides are proportional to their relative amounts. This is demonstrated for a peptide fragment of the protein B-5O(GAP-43) and for kemptide, respectively substrates for protein kinases C and A, and demonstrates the applicability of fast atom bombardment-mass spectrometry to quantitate peptides bearing post-translational modifications.Keywords
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