Time dependent effects of adriamycin and x‐ray therapy on wound healing in the rat

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Abstract
Wound healing as measured by wound breaking strength (WBS) was studied in male Fischer rats. Animals were wounded (day 0) and treated with adriamycin (ADR), X-ray therapy (XRT) or the combination of ADR + XRT. Treatments were on day -7, 0 or + 7. Animals were killed on days +14 and +21; excised wounds were subjected to uniaxial extension and the recording of WBS (grams), hydroxyproline assay and collagen fiber diameter measurements. Day 0 treatment yielded WBS averaging 46% (ADR), 41% (XRT) and 26% (ADR + XRT) of control by 21 days. Newly synthesized hydroxyproline (scar collagen) values of day 0 treated animals were 47% (ADR), 66% (XRT) and 39% (ADR + XRT) of control at 21 days. Day -7 treatment yielded similar WBS values. Withholding single agent or combined treatment until day +7 yielded WBS values comparable to control at 14 or 21 days. In this model, day 0 treatment with ADR, XRT, or ADR + XRT impaired WBS most significantly. This was supported by a diminution in newly synthesized hydroxyproline and load extension curve analysis.