The isoprostane, 8-epi-PGF2α, is accumulated in coronary arteries isolated from patients with coronary heart disease
Open Access
- 1 August 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Cardiovascular Research
- Vol. 43 (2) , 492-499
- https://doi.org/10.1016/s0008-6363(99)00108-x
Abstract
Objective: In the present study we wanted to know whether 8-epi-PGF2α, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n=23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n=19) or from healthy heart donors (controls, n=6). Methods: Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2α in the vascular intima and media. In addition, the 8-epi-PGF2α content was determined using a specific immunoassay after extraction and purification. Results: The immunohistochemical results indicated that 8-epi-PGF2α is significantly enriched in arteries from patients suffering from CHD as compared to CMP (PConclusions: From our findings we conclude that 8-epi-PGF2α is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis.Keywords
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