Trace Analysis of Chloramphenicol Residues in Egg Powders by Capillary Gas Chromatography-Electron Capture Detection
- 1 October 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 28 (10) , 501-504
- https://doi.org/10.1093/chromsci/28.10.501
Abstract
Chloramphenicol (CAP) in egg powder samples was extracted with acetonitrile by sonication. After centrifugation, the supernatant was diluted with water and de fatted with n-hexane, and cleanup was performed on a column of graphitized carbon black (GCB). Different silylation methods were compared for the derivatization of the analytes. Trimethylsilyl N,N-dimethylcerbamate produced stable di-TMS derivatives which were analyzed by capillary gas chromatography-electron capture detection (CGC-ECD) after splitless Injection. The meta isomer of the ehloramphenicol served as the internal standard for quantitation, and 80%+ mean recovery was obtained in the 0.1–10 μg/kg (ppb) concentration range.This publication has 1 reference indexed in Scilit:
- Electron-capture detection of chloramphenicol using a heptafluorobutyrate derivativeJournal of Chromatography A, 1979