A Qualitative Gas Chromatographic Analysis of Substituted 5,6-Dihydroxyindoles from the Urine of Patients with Melanoma
Open Access
- 1 August 1981
- journal article
- research article
- Published by SAGE Publications in Tumori Journal
- Vol. 67 (4) , 325-332
- https://doi.org/10.1177/030089168106700409
Abstract
A qualitative gas chromatographic analysis of trimethylsilylated ethyl acetate extracts of melanotic urine revealed 5 indolic compounds, which have been identified as substituted 5,6-dihydroxyindoles. Ethyl acetate extracts of melanotic urines at pH 2.0 contained isomeric 5-hydroxy-6-methoxy and 6-hydroxy-5-methoxy-indolyl-2-carboxylic acids which were not separable under the conditions used. A careful hydrolysis of melanotic urine with a Helix pomatia preparation followed by extraction at pH 6.5 in a nitrogen atmosphere released 3 additional indolic compounds from their conjugated form. Using gas chromatographic-mass spectrometric analysis they were identified as 5-hydroxy-6-methoxy, 6-hydroxy-5-methoxyindole and 5,6-dihydroxyindole.This publication has 13 references indexed in Scilit:
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