Imunohistochemical Localization of α-Amylase in Cotyledons of Vigna mungo Seedlings

Publisher Website
Google Scholar
Abstract
We studied the localization of .alpha.-amylase with indirect fluorescence microscopy in transversely sectioned cotyledons of Vigna mungo seedlings. Tissue sections were fixed in periodate-lysine-paraformaldehyde and treated with anti-.alpha.-amylase immunoglobulin G followed by fluorescein isothiocyanate labeled goat anti-rabbit immunoglobin G. .alpha.-Amylase appeared in the cells farthest from vascular bundles on the second day of growth and appeared gradually closer to the vascular bundles as growth progressed. The pattern of .alpha.-amylase appearance was similar in detached cotyledons, indicating that attachment of the embryonic axis has no effect on this pattern. However, in attached cotyledons, .alpha.-amylase disappeared from the regions where starch grains had been digested, but in detached cotyledons there was no disappearance of .alpha.-amylase, and digestion was slower than in intact cotyledons.