Three copies of a single recombination repeat occur on the 443 kb mastercircle of thePetunia hybrida3704 mitochondrial genome
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (18) , 7345-7357
- https://doi.org/10.1093/nar/17.18.7345
Abstract
At 443 kb, the map of Petunia hybrida line 3704 mitochondrial DNA is the largest yet produced from a dicot plant. Regions of similarity to known plant mitochondrial genes and to the chloroplast genome have been placed on a master circle. One long repeated sequence, apparently active in recombination, is present in three copies. Two copies of 6.6 kb occur in a direct orientation and are separated by 199 kb. A third truncated copy of 3.5 kb is inverted relative to the other two and is separated from the others by 99 and 145 kb. The presence of the recombination repeats predicts a multipartite molecular organization, consisting of four master circles and three subgenomic circles. Two other repeated regions were found not to be substrates for, or products of recombination. The absence of recombination at certain reiterated regions indicates that there is specificity of recombination at the recombination repeatsKeywords
This publication has 41 references indexed in Scilit:
- Molecular biology of plant mitochondriaCell, 1989
- Sequence analysis of the wheat mitochondrial atp6 gene reveals a fused upstream reading frame and markedly divergent N termini among plant ATP6 proteinsGene, 1988
- The overproduction of DNA terminase of coliphage lambdaGene, 1987
- Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric FieldScience, 1986
- Nucleotide Sequence of ATPase Subunit 6 Gene of Maize MitochondriaPlant Physiology, 1985
- Nucleotide Sequence of the F1-ATPase α Subunit Gene from Maize MitochondriaPlant Physiology, 1985
- Analysis of cosmids using linearization by phage lambda terminaseGene, 1985
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- The zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codonsCell, 1981
- The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae)Cell, 1981