Change in Small Intestinal Brush Border Membranes of Rats Following Methotrexate Administration
- 1 October 1989
- journal article
- research article
- Published by Wiley in Basic & Clinical Pharmacology & Toxicology
- Vol. 65 (4) , 269-273
- https://doi.org/10.1111/j.1600-0773.1989.tb01171.x
Abstract
Change in small intestinal brush border membranes of rats following methotrexate administration was monitored by the fluorescence spectra and polarization of the cationic fluorescent probe, safranin 0. Total protein content of brush border membranes of treated rats was less than that of control rats, whereas no significant difference in total sialic acid content of brush border membranes was observed between them. Thus, an apparent increase in the electronegative charge of brush border membrane vesicles per unit of membrane protein following methotrexate administration may possibly cause the change in fluorescence spectra and polarization of safranin 0.Keywords
This publication has 23 references indexed in Scilit:
- Effect of Methotrexate on Drug Absorption from the Rat Small Intestine in Situ and in VitroActa Pharmacologica et Toxicologica, 2009
- Effects of mitomycin C, 5-fluorouracil and cyclophosphamide on drug absorption, enzyme activities and mucosal lipid composition of intestine.Journal of Pharmacobio-Dynamics, 1987
- Effects of Salicylic Acid on the Permeability of the Plasma Membrane of the Small Intestine of the Rat: a Fluorescence Spectroscopic Approach to Elucidate the Mechanism of Promoted Drug AbsorptionJournal of Pharmaceutical Sciences, 1986
- Changes in D-glucose uptake by brush-border vesicles from small intestine of rats treated with mitomycin CBiochemical Pharmacology, 1986
- Fluorescence study on the interaction of salicylate with rat small intestinal epithelial cells: Possible mechanism for the promoting effects of salicylate on drug absorptionLife Sciences, 1985
- Aggregation of renal brush border membranes by concanavalin A and heavy metalsToxicology and Applied Pharmacology, 1982
- Changes in the mucosa of the small intestine following methotrexate administration or abdominal X‐irradiationJournal of Anatomy, 1974
- Asymmetric distribution of ouabain-sensitive ATPase activity in rat intestinal mucosaBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- The effect of oral methotrexate on the rat intestineBiochemical Pharmacology, 1966
- Cell proliferation and migration as revealed by radioautography after injection of thymidine‐H3 into male rats and miceJournal of Anatomy, 1960