Up‐regulation of ICAM‐1 expression on human dermal fibroblasts by IFN β in the presence of TNF‐α

Abstract
Unstimulated human fibroblasts show low or undetectable ICAM-1 expression. Interferon-beta (IFN-β) at concentrations of 10,100, and 1000 IU/ml in the presence of tumor necrosis factor-alpha (TNF-α) significantly increased the ICAM-I expression of fibroblasts in a dose-dependent manner. Treatment with IFN-β alone, however, did not up-regulate the ICAM-1 expression. Furthermore the attachment of peripheral blood mononuclear cells (PBMCs) to cytokine-treated fibroblasts was increased. This augmented attachment was partly inhibited by anti-ICAM-1 antibody. These results suggest that IFN-β and TNF-α may cooperatively modulate the attachment of PBMCs in the dermis

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