Quantitation of Plasma Warfarin Concentrations by High Performance Liquid Chromatography

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Abstract
A rapid, sensitive and specific high performance liquid chromatographic (HPLC) method for the quantitative analysis of warfarin in [human] plasma is described. The method involves an extraction from acidified plasma, removal of basic substances and reextraction into ether. The method is sensitive (0.06-9.0 .mu.g/ml) and precise (coefficient of variation less than 2%) and makes use of a Bondapak C-18 column. The method described is specific, accurate and convenient. It has sufficient sensitivity to measure low warfarin levels, is linear to 9 .mu.g/ml and is free of interference by common drugs. The clinical utility of this method is illustrated with 3 case reports.

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