Microbial degradation of disinfectants. I. Chlorhexidine-degrading bacteria isolated from activated sludge.

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Abstract
Aerobes of facultative anaerobes able to degrade chlorhexidine or resistant to it were isolated from activated sludge. Most of these microorganisms were Gram-negative rods. Degradation of chlorhexidine was measured by using a high-performance liquid chromatographic assay, and growth of bacteria was measured by the Kennedy and Fewson method as protein. Chlorhexidine-degrading activity of isolates could be categorized into three distinct types. Two Gram-negative, motility-positive rods were shown to utilize chlorhexidine as a sole nitrogen source for growth. On the other hands, other strains did not utilize chlorhexidine, though they were resistant to it.

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