Oxytocin Receptors in Human Uterus*
- 1 June 1974
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 38 (6) , 1052-1056
- https://doi.org/10.1210/jcem-38-6-1052
Abstract
[3H]oxytocin ([3H]OT) was bound in vitro to the 20,000 × g particulate fraction prepared from the myometrium of 2 women, 14–16 weeks pregnant. The apparent dissociation constants for oxytocin binding in preparations of the two uteri were 1.99 ± 0.18 (se) × 10−9m (n = 9) and 2.81 ± 0.26 × 10−9m (n = 10), respectively. A single class of binding sites was apparent within the range of OT concentrations examined, 0.6 to 2.2 nm. About 1.8 × 10−13 moles of OT were bound per mg of particulate protein. The competition for [3H]OT binding sites by synthetic analogues of OT was specific: [desamino]OT > [8-valine]OT ⋝ [4-threonine]OT > OT > [8-lysine]vasopressin > desaminotocinol > [4-proline]OT. The OT binding sites appear to be part of the uterine receptor because of their high affinity and ligand specificity.Keywords
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