Direct Evidence of Short-Term Cold-Induced TRH Release in the Median Eminence of Unanesthetized Rats

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Abstract
IR-TRH release in the median eminence was directly estimated in conscious rats during the first 130 min of exposure to cold (4 °C), using a push-pull cannulation. A three-fold increase in IR-TRH release was observed, with a peak of 10.00 ± 2.19 pg/15 min occurring 40 min after exposure to cold; control rats, left at 24 °C, stayed at the baseline secretion rate of 3.40 pg/15 min which was the sensitivity limit of the RIA assay.

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