High performance size exclusion chromatography of starch with dimethyl sulfoxide as the mobile phase

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Abstract
Two types of high performance size exclusion chromatographic (SEC) columns were tested for the characterization of starch samples: μ Bondagel and Aquapore. The mobile phase was dimethyl sulfoxide (DMSO) containing 0.03 M sodium nitrate maintained at 80°C. The results indicated that both μ Bondagel and Aquapore can be used to determine the relative molecular weight of starch. The exclusion limits for these columns exceed ten million. Preliminary results using μ Styragel are also reported. Examples of SEC analysis of starch, including starch with anionic groups, are given.