DNA Strand Breaking and Rejoining in Response to Ultraviolet Light in Normal Human and Xeroderma Pigmentosum Cells
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 30 (1) , 55-66
- https://doi.org/10.1080/09553007614550801
Abstract
We describe a reproducible technique for measuring DNA strand breaking and rejoining in cells after treatment with U.V.-light. Results obtained with normal human cells, xeroderma pigmentosum cells (XP, complementation group A) and XP variant cells suggest that all three of these cell-types can carry out single-strand incision with equal rapidity. However, the breaks so induced appeared to be only slowly rejoined in the XP variant cells and rejoined not at all in XP complementation group A cells. Furthermore, parental strand rejoining was inhibited by caffeine in XP variant cells but not in normal cells.This publication has 32 references indexed in Scilit:
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