Immunochemical evidence for an additional H-2 region closely linked to H-2D.

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Abstract
Anti-H-2 reagents were tested on solubilized [mouse] spleen cell preparations in combinations expected to be specific for D region products. Two different types of molecules were detected. One showed the expected reactivity with antisera to private and public specificities. An additional molecule was detected which reacted only with antisera to public specificities. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis migration patterns indicated that both products have a similar molecular size of approximately 45,000 daltons. Chemical evidence is presented for the existence of a 3rd H-2-associated gene product of 45,000 MW in addition to the classical H-2K and H-2D antigens.