Analysis of Total Platinum in Tissue Samples by Flameless Atomic Absorption Spectrophotometry. Elimination of the Need for Sample Digestion

Publisher Website
Google Scholar
Abstract
A method is described for the analysis of total Pt content in a variety of histologically distinct animal tissues [rat heart, liver, kidney, brain, testes, spleen, pancreas, lung, muscle], plasma and urine following therapeutic dosing with [the antineoplastic drug] cisplatin. Sample preparation involved only tissue homogenization. Read-out was based on non-flame atomic absorption spectrophotometry. By careful optimization of instrument parameters, sample matrix effects could be overcome, eliminating the need for sample digestion. Accordingly, 0.02-4 .mu.g of total Pt/g of sample could readily be determined without influence on absolute absorbancy readings by the environment in which cisplatin was present.