The 24-hour posttransfusion survival, oxygen transport function, and residual hemolysis of human outdated-rejuvenated red cell concentrates after washing and storage at 4 degrees C for 24 to 72 hours

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Abstract
Red cell concentrates with hematocrit values of 80 .+-. 5% were stored at 4.degree. C either in citrate-phosphate-dextrose for 22-28 days or in citrate-phosphate-dextrose-adenine-one for 35-39 days. After storage, the red cells had reduced 2,3-diphosphoglycerate and levels, and biochemical treatment with a solution called PIPA [pyruvate phosphate and adenine] was used to restore these levels. The red cells were not preserved further, but instead were washed after rejuvenation with an unbuffered sodium choloride-glucose solution, pH 5.0, for in vivo studies, and with a buffered sodium chloride-glucose-phosphate solution, pH 6.8, for comparative in vitro studies. The red cells were stored in the wash solution at 4.degree. C for 72 h after washing. Red cell recovery after washing was about 95%. Twenty-four-hour posttransfusion survival value was about 80%, and the index of therapeutic effectiveness was greater than 75%. These biochemically modified washed red cells exhibited higher than normal p50 [O2 tension at 50% saturation of Hb] values, even after 3 days of postwash storage at 4.degree. C. The units that were washed with the NaCl-glucose solution with a pH of 5.0 exhibited a greater degree of hemolysis after 3 days of postwash storages at 4.degree. C than did not units that were washed with the NaCl-glucose-phosphate solution with a pH of 6.8. Outdated red cell concentrates apparently can be biochemically treated after as long as 39 days of storage at 4.degree. C and can be kept at 4.degree. C for 3 days after washing with an unbuffered sodium chloride-glucose solution, pH 5.0, with satisfactory 24-h posttransfusion survival and O2 transport function.

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