Culture of bovine mammary epithelial cells in D-valine modified medium: Selective removal of contaminating fibroblasts

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Abstract
Bovine mammary epithelial cells were cultured in nutrient medium containing D-valine substituted for L-valine in an attempt to control fibroblast overgrowth. Contamination of epithelial cell cultures with fibroblasts was prevented in cultures maintained in D-valine but not in control cultures containing L-valine. Microscopic examination of cells cultured in the D-valine medium demonstrated similar morphology as controls after 27 days in culture. Concentration of .alpha.-lactalbumin in the D-valine medium was similar to levels found in control medium. Results suggest D-valine modified medium controls fibroblast overgrowth of cell cultures, but doesn''t impair epithelial cell morphology or function.