In Vitro Infectivity of the Autographa californica Nuclear Polyhedrosis Virus Under Various Conditions
- 1 August 1978
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 40 (2) , 493-497
- https://doi.org/10.1099/0022-1317-40-2-493
Abstract
The stability of the in vitro infectivity of the non-occluded virions (NOV) of the A. californica nuclear polyhedrosis virus (Ac-NPV) was tested. Exposure of Ac-NPV NOV to solutions ranging from pH 7.5-11.4 with molarities of 0.1-0.001 showed that infectivity was most stable in 0.001 M, pH 7.5, Tris(hydroxymethyl)aminomethane (Tris)-HCl buffer. Infectious NOV could be concentrated by precipitation with 50% saturated (NH4)2SO4 or 9% (wt/wt) polyethylene glycol 4000. Treatment of concentrated preparations with 1 M-urea did not affect infectivity or the centrifugal properties of the NOV. NOV were partially purified by centrifugation through a sucrose underlay. [Trichoplusia ni ovarian cells were used].This publication has 2 references indexed in Scilit:
- A Plaque Assay for Nuclear Polyhedrosis Viruses using a Solid OverlayJournal of General Virology, 1977
- Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization comparative infectivity, and in vitro growth studiesJournal of Virology, 1976