γ-Guanidinobutyraldehyde Dehydrogenase of Vicia faba Leaves
Open Access
- 1 November 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 76 (3) , 654-657
- https://doi.org/10.1104/pp.76.3.654
Abstract
γ-Guanidinobutyraldehyde dehydrogenase was purified 27-fold in 40% yield from extracts of Vicia faba leaves. High specificity exist only for γ-guanidinobutyraldehyde and γ-aminobutyraldehyde; the Km value was 3.4 micromolar for γ-guanidinobutyraldehyde, 25 micromolar for γ-aminobutyraldehyde, and 84 micromolar (case of γ-guanidinobutyraldehyde) for NAD, respectively. The enzyme had a molecular weight of approximately 83,000. Optimal pH and temperature for activity were 9.5 and 45°C, respectively. The enzyme was inhibited strongly by p-chloromercuribenzoate, N-ethylmaleimide, and zincon (2-carboxy-2′-hydroxy-5′-sulfoformazylbenzene).This publication has 10 references indexed in Scilit:
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