Effect of Purified Gonadotropins upon Release of Spermatozoa in the Frog, Rana pipiens
- 1 March 1955
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 88 (3) , 484-487
- https://doi.org/10.3181/00379727-88-21627
Abstract
Release of spermatozoa by the frog, Rana pipiens, after giving purified follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin and human chorionic gonadotropin (HCG) was studied. Minimal doses of these hormones which produced a response in over 75% of the animals in each group were 5 mg of FSH, 0.05 mg of LH and 25 I. U. of HCG. Prolactin did not elicit a response. Our data indicate that the release of spermatozoa in the frog can be induced by LH or a substance with an LH-like activity (HCG). The positive response to FSH is attributed by us to the contamination of the preparation with LH (2 to 3% by manufacturer''s assay). It is suggested that this rapid and sensitive response to LH might be utilized to assay the purity of FSH preparations.Keywords
This publication has 4 references indexed in Scilit:
- Use of the Male Frog (Rana Pipiens) in a Biological Pregnancy TestAmerican Journal of Obstetrics and Gynecology, 1950
- THE REACTION OF MALE FROGS TO EPINEPHRINE1Endocrinology, 1949
- AN APPRAISAL OF THE MALE NORTH AMERICAN FROG (RANA-PIPIENS) PREGNANCY TEST WITH SUGGESTED MODIFICATIONS OF THE ORIGINAL TECHNIQUE1949
- THE USE OF THE MALE NORTH AMERICAN FROG (RANA PIPIENS) IN THE DIAGNOSIS OF PREGNANCY1Endocrinology, 1948