HPLC Assay for Trimethoprim in Plasma and Urine
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 11 (11) , 967-973
- https://doi.org/10.1080/00032717808059748
Abstract
A sensitive HPLC [high performance liquid chromatographic] method for the quantitation of trimethoprim in plasma and urine was developed using fluorescence detection. Plasma (or urine) samples were made basic by the addition of 3.8N sodium hydroxide and extracted with chloroform:2-propanol (95:5). After evaporation of the organic layer, a portion of the residue was analyzed by HPLC with fluorescence detection. The minimum detectable quantity is 0.1 .mu.g/ml for this method. This method was applied to the analysis of plasma and urine obtained from subjects after a single 160 mg dose of trimethoprim.This publication has 5 references indexed in Scilit:
- The gas—liquid chromatographic analysis of trimethoprim in plasma and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1978
- Determination of trimethoprim and sulfamethoxazole (co-trimoxazole) in body fluids of man by means of high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1978
- Blood and salivary concentrations of sulfamethoxazole and trimethoprim in manJournal of Pharmacokinetics and Biopharmaceutics, 1977
- Semiautomated Spectrophotofluorometric Determination of Trimethoprim in Biological FluidsJournal of Pharmaceutical Sciences, 1973
- Spectrofluorimetric Method for the Determination of Trimethoprim in Body FluidsChemotherapy, 1969