Definition of Three Serotypes of Hantaviruses by a Double Sandwich ELISA with Biotin-Avidin Amplification System
- 1 August 1985
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 66 (8) , 1733-1740
- https://doi.org/10.1099/0022-1317-66-8-1733
Abstract
A total of 7 different isolates of Hantaviruses from 5 mammalian species and 6 geographical areas were examined by a double sandwich ELISA with biotin-avidin amplification system. Three serotypes were recognized by comparing antigen titers to homologous and heterologous antibody and blocking antibody titers to homologous and heterologous antigens. Serotype 1 included 4 strains: Hantaan virus isolated from Apodemus agrarius in South Korea; Hupei-I isolated from acute-phase serum from a patient with epidemic hemorrhagic fever (EHF) in China; SR-11 isolated from a laboratory rat associated with an EHF outbreak in Sapporo, Japan; and Tchoupitoulas isolated from a wharf rat (Rattus norvegicus) trapped in New Orleans, Louisiana, USA. Serotype 2 included 2 strains of nephropathia epidemica virus, Hallnas-I and Puumala, isolated from Clethrionomys glareolus trapped in nephropathia epidemica foci in Sweden and Finland, respectively. Serotype 3 included 1 strain, Prospect Hill-I, isolated from Microtus pennsylvanicus, trapped in Frederick, Maryland, USA.This publication has 0 references indexed in Scilit: