• 1 January 1966
    • journal article
    • research article
    • Vol. 11  (2) , 115-+
Abstract
A simple method is described for preparing mouse histocompatibility antigens of high specific activity. Material is eluted from cells in hypotonic salt without allowing cytolysis; a suitable degree of hypo-tonicity depends on the cell type. Specificities determined by H-2 and some non-H-2 alleles have been measured by inhibition of release of radioactivity from labelled target cells incubated in isoantiserum and complement. Certain tumor cells and the antigens isolated from them have aberrant histocompatibility antigen composition.