A Derivatization Procedure Suitable for HPLC Analysis of Clenbuterol

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Abstract
A precolumn derivatization method has been set up for the analysis of clenbuterol by high-performance liquid chromatography (HPLC) with UV detection. After preliminary studies, N,N-dimethylaniline was chosen as reagent against clenbuterol diazonium salt to form a diazocompound. The final derivative was separated on a reversed-phase column using a phosphate buffer—acetonitrile mixture as eluent. The reproducibility was increased using a compound homologous to clenbuterol as the internal standard. The method was applied to the analysis of clenbuterol in calf urine.