LIPID HYDROPEROXIDE ACTIVATION OF N-HYDROXY-N-ACETYLAMINOFLUORENE VIA A FREE-RADICAL ROUTE
- 1 January 1976
- journal article
- research article
- Vol. 36 (8) , 2761-2767
Abstract
Linoleic acid hydroperoxide in the presence of [bovine] methemoglobin or hematin activated the carcinogen N-hydroxy-N-acetyl-2-aminofluorene via the nitroxyl free radical intermediate into 2-nitrosofluorene and N-acetoxy-N-acetyl-2-aminofluorene. Ascorbate inhibited the activation, in which case the free radical intermediate was replaced by the ascorbate free radical. On the basis of opical kinetics, it was established that the rate of linoleic acid hydroperoxide decrease paralleled the rate of N-hydroxy-N-acetyl-2-aminofluorene decrease and also the rate of 2-nitrosofluorene increase. The stoichiometry of the reaction was such that, for every 2 linoleic acid hydroperoxide molecules consumed, two N-hydroxy-N-acetyl-2-aminofluorene molecules were oxidized and one 2-nitrosofluorene and one N-acetoxy-N-acetyl-2 aminofluorene molecule was formed.This publication has 6 references indexed in Scilit:
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