Lymphocyte‐fibroblast adhesion A useful model for analysis of the interaction of the leucocyte integrin LFA‐1 with ICAM‐1

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Abstract
The adhesion of human T lymphoblasts to ICAM‐1‐expressing normal dermal fibroblasts has been assessed as a sensitive model system for the analysis of the interaction of the leucocyte integrin LFA‐1 with its counter‐receptor ICAM‐1. Using this model system, the effects of factors known to regulate the activity of LFA‐1 have been quantitated: temperature: concentration of divalent cations; and exposure to phorbol esters. We show here that under the appropriate assay conditions, this model system represents a useful and simple alternative to the detection of leucocyte binding to purified ICAM‐1 and also has the additional advantage of permitting more sensitive quantification than is possible using the homotypic adhesion assay.