Enzyme‐Linked Immunosorbent Assay (ELISA) using Staphylococcal Protein A for the Measurement of Rabies Antibody in Various Species

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Abstract
An ELISA was developed using staphylococcal protein A linked with horseradish peroxidase for detecting IgG antibody of rabies virus in human and carnivore sera (80 human, 270 fox, 40 cat, 35 marten, 5 badger and 4 polecat sera were tested in the present work). In comparison with the serum neutralization (SN) test in cell culture, close overall agreement was obtained particularly in human and cat sera (97.5%). Two post-vaccination human sera were found positive with ELISA values of 2.16 and 2.65 IU/0.2 ml, but with SN titers < 1:10. All prevaccination human sera were found negative by both tests. Regression analysis on 30 post-vaccination human sera revealed better correlation between ELISA and SN test at serum dilution of 1 : 100 than at lower serum dilutions of 1 : 80 or 1 : 20. The correlation coefficient (r) was 0.73 (p .ltoreq. 0.0001).