Mason-Pfizer Monkey Virus Isolated From Spontaneous Mammary Carcinoma of a Female Monkey. I. Detection of Virus Antigens by Immunodiffusion, Immunofluorescent, and Virus Agglutination Techniques2

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Abstract
Antisera to Mason-Pfizer monkey (M-PM) virus prepared in rabbits and monkeys reacted specifically with M-PM virus-associated antigens in microimmunodiffusion tests producing five precipitin lines. Two of the five antigens, “d” and “e,” were released after degradation of virion with ether, freezing and thawing, or disruption by sonic vibration. These antigens diffused readily in the gel matrix, did not sediment at 92,000 × g for 7 hours, and were also present in the M-PM virus-infected monkey and human NC-37 cell cultures. Antiserum to monkey cell-derived M-PM virus caused specific agglutination of M-PM virions recovered from monkey or human cells, indicating that viral coat antigens from these two sources are similar or identical. Fluorescent conjugates, prepared with concentrated immunoglobulin, specifically stained the infected cells, with fluorescence limited to the cytoplasm.

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