COMPARISON OF AURAMINE-RHODAMINE-B AND ACRIDINE-ORANGE FOR STAINING OF ACID-FAST BACTERIA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 23  (3) , 307-317
Abstract
In 6 laboratories in Czechoslovakia and East Germany the efficiency of auramine-rhodamine B [AR] and acridine orange [AO] [short-time method] for staining acid-fast bacilli [in sputum samples from tuberculosis patients] was compared. A former comparison of AR and AO [original method] pointed out the superiority of AR, but the investigation of both methods used as short-time procedures showed significantly more acid-fast rods after using AO. The number of false positive results was somewhat higher on AR staining. The results depend on the method used and on the procedure of staining and the optical equipment, and they are essentially influenced by the experience and proficiency of the microscopist. Considering the results of both studies, AR and AO are proposed for staining acid-fast rods. For AO, the short-time method is superior to the original long-time procedure.

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