USE OF HIGH-RESOLUTION P-31-LABELED TOPICAL MAGNETIC-RESONANCE SPECTROSCOPY TO MONITOR INVIVO TUMOR METABOLISM IN RATS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (2) , 481-486
Abstract
A probe using a single-tuned solenoid coil was constructed to study in vivo metabolism of rats in a wide-bore Bruker NMR spectrometer. Transplantable rat mammary adenocarcinomas (estrogen receptor-negative) were implanted into the hind leg muscle of 8-wk-old rats. The other leg without tumor was used as a control. Tumor metabolism could be distinguished from that of surrounding muscle by the appearance of Pi and sugar phosphate resonances, reflecting tissue necrosis and increased glycolysis. Tumor growth was accompanied by an increase in the size of these peaks, and the chemical shifts of the Pi peak indicated that the intracellular pH became more acidic. Administration of methotrexate (i.v.) reversed these patterns and decreased tumor volume. Changes in the phosphocreatine peaks indicated changes in tumor volume rather than in tumor metabolism. Topical magnetic resonance not only can monitor the growth of tumors in vivo but can also be used to evaluate the efficacy of chemotherapeutic drugs.