RAPD Characterization of Poa annua L. Populations in Golf Course Greens and Fairways

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Abstract
Although identical populations of Poa annua L. may colonize golf course greens and fairways, management practices may cause strong selection pressure and cause genetic shifts in adjacent populations. The objective of our research was to assess genetic differences in adjacent P. annua populations with RAPD markers. Cores were collected from greens and fairways of three golf course holes and RAPD markers used to evaluate genetic differences between the two environments. Eighty‐six primers were screened to evaluate differences between bulk samples of greens and fairways. Nine primers produced 12 RAPD markers. Five of these were used to evaluate 54 individual plant samples from each environment. Statistical evaluation indicated significant differences (P = 0.01) between the green and fairway populations for four of the RAPD markers and among holes within fairways for three markers. These differences may indicate limited gene flow between adjacent populations of P. annua.

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