Two Phase, Two-Dimensional TLC for Fingerprinting and Confirmation Procedures

1 January 1982

journal article
research article
Published by Taylor & Francis in Journal of Liquid Chromatography

Vol. 5 (8) , 1555-1566
https://doi.org/10.1080/01483918208062851

Abstract

Two-dimensional/2D/ chromatography was first described by Kirchner in 1951. A sample applied to one corner of a 20 × 20cm plate approximately 1.5cm from each edge is developed in a mobile phase that resolves the polar constituents. It is then dried, rotated 90 degrees to the first direction and placed in a second mobile phase designed to resolve the more non-polar components. In this manner, a multitude of diverse compounds can be resolved.

Keywords

FINGERPRINTING
POLAR
DIMENSIONAL TLC
DIVERSE
CONFIRMATION PROCEDURES
MOBILE PHASE
PROCEDURES TWO
TWO DIMENSIONAL/2D/CHROMATOGRAPHY

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