Single metallic nanoparticle imaging for protein detection in cells
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Open Access
- 17 September 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 100 (20) , 11350-11355
- https://doi.org/10.1073/pnas.1534635100
Abstract
We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.Keywords
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This publication has 39 references indexed in Scilit:
- In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid MicellesScience, 2002
- Precise Nanometer Localization Analysis for Individual Fluorescent ProbesPublished by Elsevier ,2002
- Synthesis of Undecagold Labeling Compounds and Their Applications in Electron Microscopic Analysis of Multiprotein ComplexesJournal of Structural Biology, 1999
- Nanoscale Science of Single Molecules Using Local ProbesScience, 1999
- Watching DNA at WorkScience, 1999
- Single MoleculesScience, 1999
- Discrete Intensity Jumps and Intramolecular Electronic Energy Transfer in the Spectroscopy of Single Conjugated Polymer MoleculesScience, 1997
- Fluorescence intermittency in single cadmium selenide nanocrystalsNature, 1996
- Nanometre-level analysis demonstrates that lipid flow does not drive membrane glycoprotein movementsNature, 1989
- Tracking kinesin-driven movements with nanometre-scale precisionNature, 1988