Varicella-Zoster Virus Specifies a Thymidylate Synthetase
- 1 May 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (5) , 1449-1455
- https://doi.org/10.1099/0022-1317-68-5-1449
Abstract
Summary A homology search of proteins predicted from the recently reported complete DNA sequence of varicella-zoster virus (VZV) revealed that the product of gene 13 was highly homologous to eukaryotic and prokaryotic thymidylate synthetases (TSs). The VZV protein was shown to be a TS by three functional tests. Firstly, a plasmid designed to express the native protein was able to complement a strain of Escherichia coli in which the natural TS gene is deleted. Secondly, in an enzyme assay for TS, extracts of the complemented strain were capable of releasing tritiated water from 2′-deoxy[5-3H]uridylate. Thirdly, these extracts contained a protein that bound isotopically labelled 5-fluoro-2′-deoxyuridylate, a ligand specific for the active site of TS. In addition, a novel ligand-binding protein was detected in human cells infected with VZV.Keywords
This publication has 27 references indexed in Scilit:
- Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein CJournal of Virology, 1983
- Studies on identifying the folylpolyglutamate binding sites of Lactobacillus casei thymidylate synthetaseArchives of Biochemistry and Biophysics, 1982
- Comparison of Thymidine Kinase Activities Induced in Cells Productively Infected with Herpesvirus Saimiri and Herpes Simplex VirusJournal of General Virology, 1982
- Conditional thymidine auxotrophic mutants of mouse FM3A cells due to thermosensitive thymidylate synthase and their prototrophic revertants.Journal of Biological Chemistry, 1981
- XbaI, PstI, and BglII restriction enzyme maps of the two orientations of the varicella-zoster virus genomeJournal of Virology, 1981
- The primary structure of Lactobacillus casei thymidylate synthetase. III. The use of 2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine and limited tryptic peptides to establish the complete amino acid sequence of the enzyme.Journal of Biological Chemistry, 1979
- Thymidylate synthetase purified to homogeneity from human leukemic cells.Proceedings of the National Academy of Sciences, 1979
- Enzymatic basis for the selective inhibition of varicella-zoster virus by 5-halogenated analogues of deoxycytidineJournal of Virology, 1976
- An Isotopic Assay for Thymidylate Synthetase*Biochemistry, 1966
- Mutant strains of herpes simplex deficient in thymidine kinase-inducing activityVirology, 1964