Repair of Radiation-Induced DNA Double-Strand Breaks in Isolated Nuclei of Physarum polycephalum
- 1 August 1979
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 79 (2) , 368-376
- https://doi.org/10.2307/3575102
Abstract
Radiation-induced DNA double-strand breaks are rejoined in homogenates and isolated nuclei of P. polycephalum. In agreement with results obtained previously for intact plasmodia, double-strand breaks are less extensively repaired in nuclei isolated from S-phase, as compared to G2-phase cultures. A corresponding propensity of DNA present in unirradiated nuclei of S-phase plasmodia toward breakage during incubation in vitro was observed. Repair of putative single-strand breaks, as ascertained from alkaline sucrose density-gradient centrifugation analysis, was not observed. The possible relationships between these phenomena are discussed.This publication has 3 references indexed in Scilit:
- Induction and repair of strand breaks and 3′-hydroxy terminals in the DNA of mammalian cells in culture following γ-ray irradiationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965
- Morphological observations on growth and differentiation of Physarum polycephalum grown in pure cultureDevelopmental Biology, 1961