Effect of Semistarvation on Kinetic Parameters for theIn VitroAccumulation and Transport ofl-Leucine in Rat Small Intestine

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Abstract
The effects of semistarvation on the kinetics of the tissue accumulation and the transmural transport of l-leucine were assessed in vitro in the rat jejunum. The Kt calculated for the tissue accumulation was slightly reduced, but the value for transmural transport differed insignificantly after semistarvation. The Vmax’s were unanimously enhanced in semistarvation for both the accumulation and the transport, regardless of the basis of expression. Semistarvation caused a profound increase in the diffusive component of the transport when assessed using carbonyl cyanide m-chlorophenylhydrazone. Energy-dependent, active component of the transport was however even significantly greater in semistarved intestine than in fed control. These results are compatible with the notion that semistarvation improves intestinal transport capacity through increase in the Vmax, which probably correlates with ‘carrier’ population, and concomitantly in the diffusive permeability across brush border and/or intestinal wall.

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