Purification of a Fibronectin-like Protein in the Plasma of Rainbow Trout.
Open Access
- 1 January 1992
- journal article
- Published by Japanese Society of Fisheries Science in NIPPON SUISAN GAKKAISHI
- Vol. 58 (2) , 343-347
- https://doi.org/10.2331/suisan.58.343
Abstract
A cell-adhesive protein was purified from the plasma of rainbow trout Oncorhynchus mykiss by affinity chromatography on heparin-Sepharose CL-6B, followed by gel filtration on Sephacryl S-200 HR. The protein promoted the spreading of BHK cells. This activity was blocked by the synthetic Gly-Arg-Gly-Asp-Ser-Pro peptide. The purified protein showed a molecular mass of 225 kDa in sodium dodecylsulfate-polyacrylamide gel electrophoresis in both the presence and absence of 2-mercaptoethanol. The rainbow trout protein and human fibronectin have weak mutual im-munological cross-reactivity, so the former appears to be a fibronectin-like protein.Keywords
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